The power of enzymes

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The power of enzymes

Methods and Materials

· 6 beakers, 00m1 ($15)

· spoons

· Soap, cut or grated into very fine pieces (6g for each experiment)

· Biological washing powder (6g for each experiment)

· lOOml measuring cylinder ($10)

· large beakers, 500m1 ($0)

· Cotton wool ($.50)

Procedures

Cut the sample of cotton wool and roll into 6 small bundles. Stain two bundles with soil, two with egg and two with used cooking oil. Do not stain too heavily, otherwise the cleansing effect would be difficult to observe. See diagram below.

Cut the sample of cotton wool and roll into 6 small bundles. Stain two bundles with soil, two with egg and two with used cooking oil. Do not stain too heavily, otherwise the cleansing effect would be difficult to observe.

Weigh 6g of the fine soap pieces in a 500ml beaker and add 00ml of water.

Stir to dissolve the soap. Measure l00ml of the soap solution into each of three

00ml beakers.

Weigh 6g of biological washing powder in a 500 ml beaker and add 00ml of water. Stir to dissolve. Some brands of washing powders might not dissolve readily in water, in such cases a dispersion of the washing powder in water is good enough for the experiment.

Measure 100 ml of the washing powder solution into each of the three 00ml beakers.

To each of the soap solutions, add one piece of the stained cotton either - one soil stained, one egg stained or one oil stained. Do the same for the washing powder solutions.

Examine the beakers at intervals of 5 minutes, up to half an hour. And then make one final examination at the end of 1 hour.

Some questions could be asked about this activity.

· From the results obtained, do biological washing powders have better cleansing power than soap?

· What type of enzyme or enzymes could be present in the brand of washing powder you are using?

Remarks

Use different types of washing powders or different types of stains, but only vary one factor at a time; e.g. if you compare one biological washing powder with another, test at the same temperature on the same type of stain on the same material.

Other types of stains like tomato sauce, lipsticks, grease and mangosteen skin juice may also be used.

For one of the materials that you have tried out in (a), compare the action of soap with that of the biological washing powder on stained material soaked for 15 minutes in water at the following temperatures

1. 5oC (cold water from tap)

. 45oC (warm water)

. 100oC (boiling water, CAREFUL!)

Does biological washing powder work well in boiling water? Why or why not?

What has this got to do with Biotechnology?

Enzymes are the main active ingredient in the washing powder. The main method of producing the enzymes is through the culture of microorganisms on a large scale under special conditions. Man is therefore making use of microorganisms to produce the desired products. Furthermore, man is also using the product of the microorganisms to perform a service. In this case, he is using enzymes to clean the stains. This is a valuable service!

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